Figure 2. ZIKV in fluid samples from ZIKV-infected dams and their infants.

Serum and amniotic fluid samples were obtained prenatally and postnatally at different time points after infection of macaque dams with ZIKV. Samples were assayed for ZIKV RNA (Log₁₀ GE/ml) by qPCR; negative ZIKV RNA readings are not shown. Total ZIKV-specific neutralizing antibodies in serum are represented by the dilution that achieved a 50% reduction in viral plaques (PRNT₅₀ test). All PRNT₅₀ positive and negative readings are shown. The vertical dotted line indicates time of birth for offspring. All ZIKV-infected dam-infant pairs are shown. All ZIKV RNA and PRNT₅₀ measurements from the mock-infected dam-infant pairs were below the limit of detection.