FIG. 2.

(A) C3 MAb-mediated virus immunoprecipitation. HVR5-modified Ads were immunoprecipitated under nondenaturing conditions before being subjected to Western analysis with a polyclonal serum against Ad5 (see the text). In lane C, 10⁹ VP of control AE18 virus was boiled for 2 min before being loaded. Lanes 1 to 10 correspond to AdIE30, AdIE35, AdIE37, AdIE39, AdIE40, AdIE43, AdIE44, AdIE45, AdIE46, and AdIE47, respectively. Arrows a, b, and c indicate the positions of the hexon, pV, and pVI/pVII polypeptides, respectively. (B) Virus opsonization inhibits lacZ transduction of W162 reporter cells. Control virus AE18 (lane C) or the indicated HVR5-modified viruses were incubated for 1 h at 37°C in the absence (solid bars) or presence (open bars) of C3 MAb before being used for infection. The number of X-Gal-positive cells was determined after 48 h.