FIG. 3.

RGD inclusion in HVR5 enhances the transduction of cells naturally refractory to Ad5 infection. (A) Human primary aortic smooth muscle cells (Clonetics, San Diego, Calif.) were infected with control AE18 (top) or the RGD-containing virus AE57 (bottom) at an MOI of 1,000 or 10,000 VP/cell as indicated. X-Gal staining was carried out 48 h post-infection. (B) lacZ specific activity following infection with AE18 (□) or AE57 (●) at the indicated MOI. Extracts were prepared 48 h postinfection, at which time total protein and β-galactosidase activity were quantified. Data represent the means and standard deviations of duplicate experiments.