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. 2024 Jul 3;9(28):30945–30953. doi: 10.1021/acsomega.4c03949

Figure 1.

Figure 1

Schematic representation of the cloning strategy for the development of targeted vectors. The CAG promoter from the donor plasmid (pAAV-CAG-iCasp922) was replaced with an LP1 promoter. A consensus Kozak sequence (GCCGCCACC) was added before the iCasp9 transgene to generate the pAAV-LP1Kozak-iCasp9 construct. For the second plasmid, the LP1 promoter was replaced by six repeats of the E2F1 binding site (6X E2F1) and a BIRC5 promoter. Cloning resulted in two plasmids, namely, pAAV-LP1Kozak-iCasp9 and pAAV-E2F1Birc5Kozak-iCasp9.