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. 2024 Jul 18;24:251. doi: 10.1186/s12935-024-03437-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Proliferation and cell viability of MEC-1 cells during hypoxia. A The cell growth curve of MEC-1 cells (ctrl and miR-155^–/–) in normoxia (N) vs hypoxia (H). Cells were counted within 7 days in hemocytometer chamber. For statistics we compared cell numbers in MEC-1 control cells in normoxia vs in hypoxia and the same for miR-155 deficient MEC-1 cell line. B Cell viability was measured by WST-1 assay in both normoxia vs hypoxia in 24 h and 48 h time periods. C Percentage of apoptotic cells measured by flow cytometry after Annexin V/PI staining. Cells were stained in parallel in normoxia and hypoxia conditions in 24 h and 48 h time periods. D Detection of the cell cycle kinetics by BrdU labelling in normoxia vs hypoxia in 24 h and 48 h. Left graph shows percentage of BrdU/7AAD positive cells. Right graphs depict cell population in G0/G1 phase. Representative picture of dot plot is in Supplemental Figure 3. For statistics we compared percentage (populations) of MEC-1 control cells in normoxic vs MEC-1 control cells in the hypoxic conditions (the same for miR-155 deficient MEC-1 cells). All values are means ± standard deviation obtained from three independent experiments. Statistics: t-test, two tailed, paired was used (*p<0.05; **p<0.01; ***p<0.001; ****p<0.0001). Additionally, we conducted the statistical analysis of MEC-1 ctrl cells vs miR-155^–/– in normoxia and so on in the hypoxia for the whole Figure 2. The statistical significance is shown in the Supplemental Material/Tables.