Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jul 18;24:251. doi: 10.1186/s12935-024-03437-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 4 — Measurement of metabolism in MEC-1 cells in normoxia and hypoxia. For the assessment of glucose uptake in MEC-1 cells (control (ctrl) and miR-155 ^–/–) in normoxia vs hypoxia we used A Glucose Uptake-GloTM Assay and B 2-NBDG fluorescent molecule. C Shows the concentration of Lactate measured by colorimetric assay. D LDH level was measurement by LDH-Glo^™ Cytotoxicity Assay. E Pyruvate concentration was measured by Pyruvate Assay Kit. F The electron transport chain form complex I to complex III (ETC) was measured by following the protocol in [20]. G mtATP level was measured by Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit. H ATP level was measured by CellTiter-Glo^® 2.0 Cell Viability Assay. All values are means ± standard deviation obtained from three independent experiments. Statistics t-test, two-tailed, paired was used, (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)