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Workflow using high-resolution fluorespirometry to assess changes in mitochondrial membrane potential in isolated monocytes (CD14+ ) and T-cells (CD3+ ) from fresh human blood samples. Abbreviations: TMRM, tetramethylrhodamine methyl ester; SUIT, substrate-uncoupler-inhibitor titrations; ADP, adenosine diphosphate; Dig, digitonin; Mal, malate; Pyr, pyruvate; Glut, glutamate; D1–10, 10 consecutive ADP titrations.
