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. Author manuscript; available in PMC: 2024 Jul 18.
Published in final edited form as: J Vis Exp. 2024 May 24;(207):10.3791/66863. doi: 10.3791/66863

Figure 5: Blank experiments show the change in TMRM fluorescence in response to time and titrations of substrates, uncouplers, and inhibitors (SUIT).

Figure 5:

(A) Change in TMRM fluorescence in response to titration. (B) Change in TMRM fluorescence in response to time. Experiments were conducted in 0.5 mL chambers filled with Mir05 containing 1 μM TMRM. One chamber did not receive any SUIT titrations (no injection); two chambers in two different instruments received a standard suit protocol (standard injection); one chamber received the same SUIT titrations but with a delay between each injection (delayed injection).