Table 4:

Calculating mitochondrial membrane potential (mMP) from sample experiment

	Sample TMRM (μM)	Average background ratio		Experiment background (μM)		Background corrected mMP		FCCP corrected mMP
Source/Calculation	From O2K Experiment	Calculated from blank experiments	Multiply ”Pre-sample" by the background ratio		Subtract TMRM background - sample		Subtract by FCCP mMP value
Pre-sample	0.859	1	x 0.859 =	0.859	− 0.859 =	0
CELL	0.709	0.91	x 0.859 =	0.782	− 0.709 =	0.073	− (−0.102) =	0.175
DIG	0.711	0.86	x 0.859 =	0.739	− 0.711 =	0.028	− (−0.102) =	0.13
SUCC	0.552	0.838	x 0.859 =	0.72	− 0.552 =	0.168	− (−0.102) =	0.27
MPG	0.51	0.8	x 0.859 =	0.687	− 0.510 =	0.177	− (−0.102) =	0.279
D1	0.505	0.786	x 0.859 =	0.675	− 0.505 =	0.17	− (−0.102) =	0.272
D2	0.501	0.776	x 0.859 =	0.666	− 0.501 =	0.165	− (−0.102) =	0.267
D3	0.504	0.769	x 0.859 =	0.66	− 0.504 =	0.157	− (−0.102) =	0.259
D4	0.51	0.758	x 0.859 =	0.651	− 0.510=	0.141	− (−0.102) =	0.243
D5	0.52	0.753	x 0.859 =	0.647	− 0.520 =	0.127	− (−0.102) =	0.229
D6	0.534	0.747	x 0.859 =	0.642	− 0.534 =	0.108	− (−0.102) =	0.21
D7	0.543	0.741	x 0.859 =	0.636	− 0.543 =	0.093	− (−0.102) =	0.195
D8	0.557	0.739	x 0.859 =	0.635	− 0.557 =	0.077	− (−0.102) =	0.179
D9	0.575	0.743	x 0.859 =	0.638	− 0.575 =	0.063	− (−0.102) =	0.165
D10	0.592	0.742	x 0.859 =	0.637	− 0.592 =	0.046	− (−0.102) =	0.148
OMY	0.59	0.746	x 0.859 =	0.641	− 0.590 =	0.05	− (−0.102) =	0.152
FCCP	0.738	0.74	x 0.859 =	0.636	− 0.738 =	−0.102	− (−0.102) =	0