Skip to main content
. 2024 Mar 28;14(4):213–224. doi: 10.4103/mgr.MEDGASRES-D-23-00036

Figure 7.

Figure 7

Effects of O3-RI on NLRP3 inflammasome in hippocampal CA1 and CA3 regions of SD mice using immunohistochemical staining.

Note: (A, B) Representative microphotographs of NLRP3 inflammasome in hippocampal CA1 (A) and CA3 (B) areas. Compared with the Ctrl group, the expressions of NLRP3, ASC and Caspase-1 in the hippocampus CA1 and CA3 regions of mice in the SD group were significantly increased, and this abnormal phenomenon was gradually improved in the two O3-RI treatment groups. Scale bar: 100 μm. (C–E) NLRP3, ASC and Caspase-1 immunopositivity in hippocampal CA1 area. (F–H) NLRP3, ASC and Caspase-1 expression in hippocampal CA3 area. Data are expressed as mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way analysis of variance, followed by the Tukey's multiple comparison test). Ctrl: Control; IOD: integrated option density; ns: no significance; O3-RI: ozone rectal insufflation; O3-RI-H: high-concentration O3-RI; O3-RI-L: low-concentration O3-RI; SD: sleep deprivation.