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Native-PAGE for the evaluation of ABD affinity to HSA. Lane 1, protein marker; lane 2, the upper sample of Amicon filter after centrifuging after a 2-h incubation; lane 3, the mixed sample of IL-1Ra-ABD and HSA after a 30-min incubation; lane 4, the flow-through of Amicon filter after centrifuging after a 2-h incubation. PAGE, polyacrylamide gel electrophoresis; ABD, albumin binding domain; HSA, human serum albumin; IL-1Ra, interleukin 1 receptor antagonist.
