Table 1. Primer information used for PCR analysis.
| Pathogen | Target genes | Sequences (5′–3′) | Sizes (bp) |
Annealing temp./Time |
|---|---|---|---|---|
| Anaplasma phagocytophilum | 16S rRNA | TCCTGGCTCAGAACGAACGCTGGCGGC | 1,433 | 50°C/30 s |
| AGTCACTGACCCAACCTTAAATGGCTG | ||||
| GTCGAACGGATTATTTTTATAGCTTGC | 926 | 56°C/30 s | ||
| CCCTTCCGTTAAGAAGGATCTAATCTCC | ||||
| Bartonella spp. | ITS | TTCAGATGATGATCCCAAGC | 639 | 55°C/30 s |
| AACATGTCTGAATATATCTTC | ||||
| CCGGAGGGCTTGTAGCTCAG | 499 | 55°C/30 s | ||
| CACAATTTCAATAGAAC | ||||
| Borrelia spp. | ospA | GGGAATAGGTCTAATATTAGCC | 665 | 42°C/60 s |
| CACTAATTGTTAAAGTGGAAGT | ||||
| GCAAAATGTTAGCAGCCTTGAT | 392 | 56°C/60 s | ||
| CTGTGTATTCAAGTCTGGC | ||||
| Coxiella burnetii | IS1111 | TATGTATCCACCGTAGCCAGTC | 687 | 54°C/30 s |
| CCCAACAACAACCTCCTTATTC | ||||
| GAGCGAACCATTGGTATCG | 203 | 54°C/30 s | ||
| CTTTAACAGCGCTTGAACGT | ||||
| Leptospira interrogans | rpoB | GTTCCAACATGCAACGYCAR | 1,649 | 52°C/60 s |
| GTTGAAGGATTCRGGRATAC | ||||
| TYATGCCKTGGGAAGGWTAC | 1,023 | 56°C/30 s | ||
| GCATRTCRTCKGACTTGATG | ||||
| SFTSV | S | CATCATTGTCTTTGCCCTGA | 461 | 52°C/40 s |
| AGAAGACAGAGTTCACAGCA | ||||
| AAYAAGATCGTCAAGGCATCA | 346 | 55°C/40 s | ||
| TAGTCTTGGTGAAGGCATCTT |
*SFTSV: severe fever with thrombocytopenia syndrome virus