Figure 1: Schematic overview of the mouse study and vitamin A diet intervention.

At P21, we fed cohorts of WT and Rbpr2^−/− mice with a vitamin A sufficient (VAS) or vitamin A deficient (VAD) diet. At the 3-month (early time point analysis) and 6-month time point (late time point analysis), non-invasive tests for retinal function and integrity were performed. Ocular and multiple non-ocular tissues were harvested and subjected to High performance liquid chromatography (HPLC) analysis to quantify all-trans ROL and retinoid concentrations.