Figure 8: Cone Photoreceptor functional analysis by Electroretinogram.

Photopic ERG of WT and Rbpr2^−/− mice fed with either a VAS or VAD diet and stimulated with Green, Red, White, or Blue wavelength light intensities series showing the response curves, at 3-months of age (A-D) or 6-months of age (A’-D’). IHC staining for cone opsin (Opn1mw) in photoreceptor OS in green and mislocalization in IS was analyzed by the threshold in Red, Outer nuclear layer (ONL) stained with DAPI in Blue and merged images showing the localization of mislocalized cone opsins (E). Threshold-based quantification of mislocalized cone opsin in the IS of retinas from WT and Rbpr2^−/− mice fed different vitamin A diets (F).