Figure 5. Characterization of the hydrolytic capabilities of the condenzyme in cells and their biological implications.

A, Schematic illustration of the dephosphorylation of ATP into ADP, AMP, and adenosine.

B, Mass spectroscopy assay analysis of the in vitro hydrolysis of ATP by the RLP condensates into ADP, AMP, adenosine, adenine, and carbohydrates.

C, Cellular ATP quantification assay analysis of the change of ATP level in cells with and without condensates at different time point.

D, Mass spectroscopy assay analysis of the cellular ATP, ADP, AMP, and adenosine level in cells with or without condensates.

E, RNA-seq assay analysis of the difference in ATP dephosphorylation-related gene expression in cells with or without RLP condensates. N=2 independent experiments.