Table 3.

Measurements of membrane tubule diameters using negative stain Electron Microscopy (EM) in the presence of endosomal protein complexes in the presence of physiological lipid and cargo motifs.

Purified protein complexes	Lipid	Cargo motif	Number of tubules counted (n)	Diameter (nm)
SNX27	PI(3)P	PDZbm	20	38.0 ± 5.0
SNX27 + Retromer	PI(3)P	PDZbm	50	80.0 ± 6.0
SNX27 + Retromer	Folch I	PDZbm	No tubules detected	N/A
ESCPE-1	Folch I	None	50	55.0 ± 6.0
ESCPE-1	Folch I	CI-MPR	50	53.0 ± 5.0
ESCPE-1	PI(3)P	CI-MPR	No tubules detected	N/A
ESCPE-1 + SNX27	Folch I	CI-MPR / PDZbm	50	58.0 ± 5.5
ESCPE-1 + Retromer	Folch I	CI-MPR	Tubules rarely detected (5)	50.0 ± 5.0
ESCPE-1 + SNX27 + Retromer	Folch I	CI-MPR / PDZbm	50	55.0 ± 4.0
ESCPE-1 + SNX27	PI(3)P	CI-MPR / PDZbm	Tubules rarely detected	N/A
ESCPE-1 + Retromer	PI(3)P	CI-MPR	Tubules rarely detected	N/A
ESCPE-1 + SNX27 + Retromer	PI(3)P	CI-MPR / PDZbm	50	53.0 ± 5.2
ESCPE-1 + SNX27 + Retromer + VARP	PI(3)P	PDZbm	50	69.0 ± 3.5