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. 2024 May 30;4(7):998–1013. doi: 10.1038/s43587-024-00631-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, Dot plot visualizing the cell-type-specific expression pattern of housekeeping genes (blue) and known (red) and newly identified (black) gene markers for each major tissue. Housekeeping genes rpl-32 and pmp-3 show pan-cell expression. Dot size corresponds to the percentage of cells within a specific tissue expressing the marker gene, and the color intensity indicates the average expression level of the gene across the tissue. b, Heatmaps showing the expression levels of the tissue-specific TFs (right) with the corresponding enrichment scores of their target genes (left) in each tissue. c,d, Heatmaps of tissue-specific functional modalities selected based on the enrichment scores using InterPro (c) and KEGG (d) pathway analyses. cNMP-bd_dom, cyclic nucleotide-binding domain; CNNM, cyclic nucleotide-binding domain; F-box_dom, F-box domain; Histone_H2A_C, histone H2A, C-terminal domain; Neur_chan_lig-bd, neurotransmitter-gated ion-channel ligand-binding domain; SAM, sterile alpha motif domain; SSD, sterol-sensing domain; T_SNARE_dom, target SNARE coiled-coil homology domain; ThiF_NAD_FAD-bd, THIF-type NAD/FAD binding fold; Znf_CCHC, zinc finger, CCHC type.