Extended Data Fig. 6. Structural comparison and analysis of the Spc98^NHD.

a, Structures of the N-terminal Helical Domains (NHD) of Spc98 and homologs, without (left) and with (right) Mzt1 bound. The S. cerevisiae genome does not contain the mzt1 gene. Structurally comparable helices are annotated with A, B and C to highlight similarities between Spc98^NHD and functionally equivalent proteins in other organisms. A direct overlay between the ABC helices of Spc98^NHD and GCP3^NHD is shown in the middle. GCP3^NHD and GCP6^NHD have very similar folds. b, Alphafold2 pLDDT PAE scores of the best trimeric Spc98^NHD prediction. c, Bottom view of the γTuRC map shows bridging connections between Spc98 modules and the luminal density.