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. 2024 Apr 12;31(7):1134–1144. doi: 10.1038/s41594-024-01281-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a, Consensus cryo-ET map of the γTuRC with a fitted molecular model of the γTuRC shows that the secondary structure and path of Spc110^NCC and Spc110^CM1 are resolved and in agreement with previous reconstituted structures¹⁸. b, At lower threshold the sub-tomogram average shows that Spc110^NCC densities continue downwards until they converge on a single large stalk density. The map was blurred with a B-factor of 400 for visualization purposes. c, 3D classification of MTs was used to separate MT species according to protofilament numbers, following the MiRP procedure⁶⁸. Representative slices through reference volumes used during 3D classification of the microtubule sub-tomogram volumes. 97% of spindle microtubules contain 13 protofilaments.