FIG. 3.

Capping of CD46 by anti-CD46 antibodies in the absence of modulation. HeLa cells were detached from the plastic dish prior to incubation with anti-CD46 antibody 13/42 for 30 min on ice (A), 30 min at 37°C (B), and 60 min at 37°C (C) and analyzed in a fluorescence microscope to visualize the formation of caps. In addition, CD46 expression on aliquots of the cells was quantified by flow cytometry (D). The average CD46 signals on the differentially treated cell populations after 0 min (=control on ice), and 30, 60, and 240 min at 37°C were similar.