FIG. 5.

Contact-mediated CD46 downregulation and transfer of viral proteins to HeLa cells. A HeLa cell monolayer was overlaid with persistently MV-Edm-infected U937-p cells for 3 h (cell ratio of 1:1). After contact, U937-p cells were removed by intensive washing with PBS from the HeLa cell monolayer, and the contacted HeLa (D to F) and U937-p (J to L) cells were separately analyzed by flow cytometry. Control cells were untreated HeLa (A to C) and U937-p (G to I) cells. The signals for the surface expression of CD46 (A, D, G, and J) and MV H (B, E, H, and K) and the expression of MV N after permeabilization (C, F, I, and L) are shown. Numbers in the panels represent mean fluorescence intensities.