Fig. 3.

Characterizations of the co-immobilized enzymes. (a), Thermo-stability of the free and co-immobilized enzymes; (b), pH stability of the free and co-immobilized enzymes; (c), Solvent tolerance of co-immobilized enzymes; (d), Reusability of the enzymes co-immobilized on various carriers. Reaction conditions: NAD⁺ (1 mM), glucose (100 mM), Tris-HCl (including 1 M NH₄Cl) buffer (50 mM, pH 9.0 for LXTE-700s, or pH 9.5 for LXTE-706), immobilized enzymes (200 mg), 1a (10 mM), 30 °C, total volume 1 mL, incubation time: 2 h