FIG. 3.
(A) Analysis of [3H]uridine-labeled intracellular RNA isolated 12 h after infection or transfection from mock-transfected cells (lane M), cells infected with EAV (lane I), or cells transfected with EAV030F RNA (lane F) or EAV030H RNA (lane H). The RNAs were separated in a denaturing 1% agarose gel. (B) Schematic representation of the conversion of RIs into RFs by RNase treatment. (C) Analysis of [3H]uridine-labeled RF RNAs obtained 12 h postinfection or transfection from cells infected with EAV (lane I) and cells transfected with EAV030F RNA (lane F) or EAV030H RNA (lane H). The RFs corresponding to EAV RNA1 to RNA7 are indicated with I to VII, respectively. The RF RNAs were separated in a nondenaturing 1% agarose gel.