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. 2024 Jul 18;15:6073. doi: 10.1038/s41467-024-50493-9

Fig. 2. BONCAT identifies novel secreted rickettsial factors (Srfs) that are structurally diverse and variably conserved.

Fig. 2

R. parkeri protein hits identified from infected host cytoplasmic lysates using BONCAT include proteins that had been experimentally validated as secreted effectors in previous studies (light blue), autotransporter proteins (dark blue), and the novel secreted effectors SrfA–G (beige). Hit calling is described in the “Methods” section. Protein lengths, putative domains, and structural motifs are indicated for SrfA–G. CC coiled coil, ARMR armadillo-like repeats, TM transmembrane helix, PPR pentapeptide repeats, DUF Pfam domain of the unknown function. BLAST E-values were computed to evaluate the similarity between R. parkeri SrfA–G and putative homologs in select members of the Rickettsia genus. Species lacking a detected Srf homolog were left blank. co R. conorii, ri R. rickettsii, pe R. peacockii, mt R. montanensis, Ip Rickettsia endosymbiont of Ixodes pacificus, mn R. monacensis, bu R. buchneri, fe R. felis, ak R. akari, pr R. prowazekii, ty R. typhi, he R. helvetica, ca R. canadensis, be R. bellii, Bt Rickettsia endosymbiont of Bemisia tabaci MEAM1, Pp Rickettsia endosymbiont of Pyrocoelia pectoralis. Source data are provided as a Source Data file.