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. 2024 Jul 18;7(9):e202402893. doi: 10.26508/lsa.202402893

Figure S11. Effects of PY-60 and XAV9393 on patterned keratinocytes.

Figure S11.

(A) Immunofluorescent images of patterned keratinocytes in a high-density area with or without PY-60. Hoechst 33342 (blue); Yes-associated protein (YAP) (green). Scale bar: 50 μm. (B) Comparison of the nuclear YAP intensity of keratinocytes with or without PY-60. The nuclear YAP intensity was quantified using the nuclear and cytosol intensity ratio. N = 6 for each group. (C) Immunofluorescent images of patterned keratinocytes in a high-density area with or without PY-60. Hoechst 33342 (blue); ANKRD1 (green). Scale bar: 50 μm. (D) Comparison of the nuclear ANKRD1 intensity of keratinocytes with or without PY-60. The nuclear ANKRD1 intensity was quantified using the nuclear and cytosol intensity ratio. N = 6 for each group. (E) Immunofluorescent images of keratinocytes with or without PY-60 treatment. Nuclei are labeled with Hoechst 33342. Scale bar: 500 μm. (F) Pattern index with or without PY-80 treatment. N = 8 for each group. (G) Immunofluorescent images of patterned keratinocytes in a high-density area with or without XAV939. Hoechst 33342 (blue); YAP (green). Scale bar: 50 μm. (H) Comparison of the nuclear YAP intensity of keratinocytes with or without XAV939. The nuclear YAP intensity was quantified using the nuclear and cytosol intensity ratio. N = 6 for each group. (I) Immunofluorescent images of patterned keratinocytes in a high-density area with or without XAV939. Hoechst 33342 (blue); ANKRD1 (green). Scale bar: 50 μm. (J) Comparison of the nuclear ANKRD1 intensity of keratinocytes with or without XAV939. The nuclear ANKRD1 intensity was quantified using the nuclear and cytosol intensity ratio. N = 6 for each group. (K) Immunofluorescent images of keratinocytes with or without XAV939 treatment. Nuclei are labeled with Hoechst 33342. Scale bar: 500 μm. (L) Pattern index with or without XAV939 treatment. N = 8 for the vehicle group and N = 7 for the XAV939 treatment group. All data are presented as mean values and were analyzed with two-tailed Mann–Whitney U tests. *P < 0.05. **P < 0.01. ***P < 0.001.