FIG. 1.
Bulk culture recognition of Hantaan virus proteins. PBMC (2 × 106 to 5 × 106/well) were stimulated for 7 to 14 days with a gamma-irradiated Hantaan virus as described in Materials and Methods. Target cells were autologous BLCL infected with recombinant vaccinia viruses expressing one or more Hantaan virus proteins. Vaccinia recombinants used to infect targets (shown on the x axis) express individual Hantaan virus proteins (vac-G1, -G2, and -N) or a combination of G1, G2, and N (vac-G1+G2+N). Lysis of targets infected with wild-type vaccinia virus served as a negative control. E/T ratio, 80. Data from a representative experiment are shown.