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. 2023 Aug 11;61:133–149. doi: 10.1016/j.jare.2023.08.007

Fig. 4.

Fig. 4

CPT2 overexpression decreases LCAC levels in vitro and in vivo. (A-B) CPT1 and CPT2 activity were determined in isolated mitochondria extracted from ischemic penumbra in tMCAO mice and corresponding area in sham mice. Data are presented as mean ± SD (N = 7 each group). *p < 0.05, ***p < 0.001, one-way ANOVA followed by Dunnett's multiple comparisons test. (C) Relative abundance of the AcCa(C16), AcCa(C18), AcCa(C18:1) and LCAC (the sum of AcCa(C16), AcCa(C18), and AcCa(C18:1)) in ischemic penumbra after 3 days in tMCAO mice or corresponding area in sham mice. Data are presented as mean ± SD (N = 5–6 each group). *p < 0.05, **p < 0.01, one-way ANOVA followed by Tukey's multiple comparisons test. (D, G, J) Microglia (D), neuron (G), astrocyte (J) cultures were immunostained for Iba-1, MAP2, GFAP to label microglia, neuron, astrocyte, respectively. scale bar = 50 μm. (E, H, K) PCA plot depicting separation of the control and OGD/R microglia (E), neuron (H) and astrocytes (K) utilizing the first two components of the PCA model. (F, I, L) Heatmap of detected AcCa in microglia (F), neuron (I) and astrocytes (L). (M) Relative abundance of the AcCa(C16), AcCa(C18), AcCa(C18:1) and LCAC (the sum of AcCa(C16), AcCa(C18), and AcCa(C18:1)) in the control and OGD/R astrocytes. Data are presented as mean ± SD (N = 5 each group). *p < 0.05, **p < 0.01, ***p < 0.001, unpaired, two-tailed t test. (N) Relative abundance of the AcCa(C16), AcCa(C18), AcCa(C18:1) and LCAC (the sum of AcCa(C16), AcCa(C18), and AcCa(C18:1)) in the OGD/R astrocytes with Ad-GFP or Ad-CPT2 infection. Data are presented as mean ± SD (N = 5–6 each group).