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Agarose gel of HIV polytope PCR products from cDNA (lane B) and viral DNA (lane E) derived from rVV.HIV.pt-infected cells. Lanes A and F, 1-kb markers (Gibco BRL, Gaithersburg, Md.); lane B, RT-PCR of the HIV polytope mRNA; lane C, RT-PCR control without RT; lane D, PCR control with water as template; lane E, PCR of viral DNA. Molecular size markers indicated by arrowheads are (from the top) 394, 344, 298, and 220 bp.
