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. 2024 Mar 21;22(8):2145–2156. doi: 10.1111/pbi.14335

Figure 2.

Figure 2

Effect of in‐frame deletion or substitution mutation of E1 and E1Lb on flowering time under long‐day (16 h light) condition in GH. (a) 38‐day‐old plants of 06KG218440 (E1‐WT, E1Lb‐WT); SYKE00D (E1‐Sub, E1Lb‐WT); and SYKE00A (E1‐Lof, E1Lb‐WT). Edited lines SYKE00A and SYKE00D showed obvious early flowering compared to 06KG218440, and flowering time of SYKE00D was intermediate between those of SYKE00A and 06KG218440. Each bar represents the mean of five plants. Error bars represent standard errors (**P < 0.01). (b) 30‐day‐old plants of E1Lb‐Inf and E1Lb‐Lof genotype of the E1‐Lof gene background after emergence. E1‐Lof combining either the E1Lb‐Inf (SYKE009) or the E1Lb‐Lof mutation (SYKE008) showed earlier flowering compared to both 06KG218440 and single E1‐Lof mutation (SYKE00A), but the E1Lb‐Inf mutation had less of an additive effect than E1Lb‐Lof mutation on early flowering. Detailed data for flowering times are provided in Table S1. Each bar represents the mean of five plants. Error bars represent standard errors (**P < 0.01). Flowering time is from VE (emergence) to R1 (first open flower in main stem). Sub is the abbreviation for substitution, Inf is the abbreviation for in‐frame deletion, and Lof is the abbreviation for loss of function. 06KGWT is the abbreviation for the 06KG218440 wild type.