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Mutations introduced into PrV. (A) Diagram of the PrV genome. It is divided into a unique long (UL) and unique short (US) region by inverted repeats (open rectangles) which bracket the latter. (B) BamHI restriction fragment map. (C) Locations of the glycoprotein genes which had been mutated as demonstrated in panel D. Relevant restriction sites: B, BamHI; Bs, BstEII; D, DdeI; Dr, DraI; N, NlaIII; K, KpnI; S, SalI; Sp, SphI; St, StuI.
