One-step growth analysis of PrV-1112 (⧫), PrV-gE/I− (■), PrV-gM− (▴), or PrV-gE/I/M− (X) in nontransfected (A), gM-expressing (B), or gE/I-expressing (C) RK13 cells. At the indicated times after infection, supernatant and cells were harvested and titrated on RK13 cells, and titers were summed. Average values and standard deviation of two independent experiments are shown.