Fig 1. Experimental approach for identifying EMS derived L. monocytogenes mutants with increased tolerance to benzalkonium chloride.

Experimental approach used for chemical mutagenesis, mutant screening for increased tolerance to benzalkonium chloride, antibiotic susceptibility determination and whole genomic sequencing of mutants with increased tolerance to benzalkonium chloride. Portions of this figure utilized images from Clker.com, licensed under Creative Commons CC0 1.0, and Servier Medical Art, licensed under Creative Commons Attribution 4.0.