Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Arabidopsis thaliana) | Col-0 | ther | wild-type ecotype | |
| Strain, strain background (Nicotiana benthamiana) | WT | Lab stock | Wild-type strain | |
| strain, strain background (Escherichia coli) | BL21(DE3) pLPP | Amid Biosciences | Cat#: BLPP-201 | Co-expression of lambda phosphatase |
| Strain, strain background (Escherichia coli) | BL21(DE3)-V2R-pACYC LamP | PMID: 20436473 | Co-expression of lambda phosphatase |
|
| Strain, strain background (Agrobacterium tumefaciens) | GV3101 | Lab stock | ||
| Strain, strain background (Arabidopsis thaliana) | efr-1 | PMID: 16713565 | ||
| Genetic reagent (Arabidopsis thaliana) | EFR-GFP | This paper | See Materials and methods |
|
| Genetic reagent (Arabidopsis thaliana) | F761H#2 | This paper | See Materials and methods |
|
| Genetic reagent (Arabidopsis thaliana) | F761H#7 | This paper | See Materials and methods |
|
| Genetic reagent (Arabidopsis thaliana) | Y836F#1 | This paper | See Materials and methods |
|
| Genetic reagent (Arabidopsis thaliana) | Y836F#4 | This paper | See Materials and methods |
|
| Genetic reagent (Arabidopsis thaliana) | F761H/Y836F#1 | This paper | See Materials and methods |
|
| Genetic reagent (Arabidopsis thaliana) | F761H/Y836F#5 | This paper | See Materials and methods |
|
| Genetic reagent (Arabidopsis thaliana) | SSAA#2 | This paper | See Materials and methods |
|
| Genetic reagent (Arabidopsis thaliana) | SSAA#7 | This paper | See Materials and methods |
|
| Genetic reagent (Arabidopsis thaliana) | F761H/SSAA#2 | This paper | See Materials and methods |
|
| Genetic reagent (Arabidopsis thaliana) | F761H/SSAA#7 | This paper | See Materials and methods |
|
| Sequence-based reagent | EFR-f | This paper | PCR primer | atcgGAAGACaaAATGA AGCTGTCCTTTTCACTTG |
| Sequence-based reagent | EFR Y836F-f | This paper | PCR primer | ggagtTtctgcacgtt cattgtcatgaccctgta |
| Sequence-based reagent | EFR SSAA-f | This paper | PCR primer | Gtttgctgctgctggt gtcagaggcaccat |
| Sequence-based reagent | EFR F761H-f | This paper | PCR primer | acgatgtcgtatacccttgt gggtttcacattccgccata |
| Sequence-based reagent | EFR F761M-f | This paper | PCR primer | tacgatgtcgtatacccttc atggtttcacattccgccata |
| Sequence-based reagent | EFR V845F-f | This paper | PCR primer | gcttaatatcacagtgagc gaaagggtcatgacaatgaacgtg |
| Sequence-based reagent | EFR L743F-f | This paper | PCR primer | gctttaggaggttgaaaac tttaaccgcgacgagttta |
| Sequence-based reagent | EFR L873E-f | This paper | PCR primer | ctcagGAGctctataaata cgatcgagaatcctttcta |
| Sequence-based reagent | EFR dNLLKH-f | This paper | PCR primer | taaagttttgggagcgacga aaagctttatggcggaatgtg |
| Sequence-based reagent | EFR D849N-f | This paper | PCR primer | cactgtAatattaagcca agcaacattcttctagacgat |
| Sequence-based reagent | BAK1-f | This paper | PCR primer | atcgGAAGACaaAATGGA ACGAAGATTAATGATCC |
| Sequence-based reagent | BAK1 Y403F-f | This paper | PCR primer | cttgcgtttttacatgat cattgcgacccaaaga |
| Sequence-based reagent | BAK1 I338H-f | This paper | PCR primer | gagatgCAtagtatggcg gttcacagaaacttgct |
| Sequence-based reagent | BAK1 D416N-f | This paper | PCR primer | catcgaAatgtgaaagctg caaatattttgttggatgaag |
| Sequence-based reagent | EFRprom-f | This paper | PCR primer | atcgGgtctctTACGCGTCTC aGTAGatctagacgat taagtaattgagca |
| Sequence-based reagent | BAK1prom-f | This paper | PCR primer | atcgGgtctctTACGCGTCTCa CCTAtgtcgtgaaaagggcac |
| Sequence-based reagent | XIIa2_Tmicd-f | This paper | PCR primer | atcgGCTCTTCgAAGGTT CTTCTACCGGTTCTGTTAT |
| Sequence-based reagent | XIIa3_Tmicd-f | This paper | PCR primer | atcgGCTCTTCgAAGG TTGCGATTGGGGTCA |
| Sequence-based reagent | XIIa4_Tmicd-f | This paper | PCR primer | atcgGCTCTTCgAAGATAA TCACCATTTGTGTCAGTG |
| Sequence-based reagent | XIIa5_Tmicd-f | This paper | PCR primer | atcgGCTCTTCgAAGGTT GTGATTGGAGTTAGCGT |
| Sequence-based reagent | XIIa6_Tmicd-f | This paper | PCR primer | atcgGCTCTTCgAAGGTTG CAATTTTAGTAAGCATAGG |
| Sequence-based reagent | FLS2_Tmicd-f | This paper | PCR primer | atcgGCTCTTCgAAGGTCA TCCTGATTATTCTTGGATCA GCCGCGGCaCTTCTTCTTGTCCTG |
| Sequence-based reagent | AlXIIa_D850N-f | This paper | PCR primer | atcgGCTCTTCAGTa ATATTAAGCCAAGCAACG |
| Sequence-based reagent | BrXIIa_D846N-f | This paper | PCR primer | atcgGCTCTTCAGTaA TCTTAAGCCAAGCAAC |
| Sequence-based reagent | GmXIIa_D829N-f | This paper | PCR primer | atcgGCTCTTCAGTaATA TTAAGCCAAGCAACATT |
| Sequence-based reagent | PtXIIa_D848N-f | This paper | PCR primer | atcgGCTCTTCAGT aatctgaagccaagcaa |
| Sequence-based reagent | SlXIIa_D883N-f | This paper | PCR primer | atcgGCTCTTCAGTaATAT AAAACCACAGAACATTCT |
| Sequence-based reagent | XIIa2_D803N-f | This paper | PCR primer | atcgGCTCTTCAGTaATC TCAAACCGAGCAATATCC |
| Sequence-based reagent | XIIa3_D838N-f | This paper | PCR primer | atcgGCTCTTCACaATCT TAAGCCAAGCAACATACT |
| Sequence-based reagent | XIIa4_D856N-f | This paper | PCR primer |
atcgGCTCTTCAGTaATATT AAGCCAAGCAATATTCTACTA |
| Sequence-based reagent | XIIa5_D839N-f | This paper | PCR primer | atcgGCTCTTCAGCaA TCTTAAGCCAAGCAACGT |
| Sequence-based reagent | XIIa6_D840N-f | This paper | PCR primer | atcgGCTCTTCAGCaATC TCAAGCCAAGCAACG |
| Sequence-based reagent | FLS2icd_D997N-f | This paper | PCR primer | atcgGCTCTTCAGTaATC TGAAGCCAGCTAATATACT |
| Sequence-based reagent | FLS2_D997N-f | This paper | PCR primer | GTTCATTGTaATCTGAAGC CAGCTAATATACTCCTTGACA |
| Sequence-based reagent | EFR_ecto-f | This paper | PCR primer | atcgGGTCTCaAATGAAG CTGTCCTTTTCACTTG |
| Sequence-based reagent | EFR_SapIdom-f | This paper | PCR primer | GTTATGAgGAGCTTCAT AGTGCAACAAGTCGCTTC |
| Sequence-based reagent | EFR_Esp3Idom-f | This paper | PCR primer | TTCCCGTgTCTTTCGGG AAGCTTTTGAACTTGC |
| Sequence-based reagent | ccdB cassette-f | This paper | PCR primer | atgcGGTCTCAGAAGgGAAG AGCAAAGCTGAACGA GAAACGTAAAAT |
| Sequence-based reagent | pET28_lin-f | This paper | PCR primer | tgagatccggctgctaa |
| Sequence-based reagent | pETGG cloning cassette-f | This paper | PCR primer | AGAAGGAGATATACCAATGAGAGACCAAAGCTGAA |
| Sequence-based reagent | P641-BsaI_mut1-f | This paper | PCR primer | agagacCaaagctgaacga gaaacgtaaaatgatataaata |
| Sequence-based reagent | P641-BsaI_mut2-f | This paper | PCR primer | gccagtGgtctcttc tggtcgtgactggg |
| Sequence-based reagent | 6xHis-FKBP-f | This paper | PCR primer | atcgCGTCTCtTACGGGTCT CaAATGggcagcagccatcatcatc atcatcacagcagcggcGGA GTGCAGGTGGAAAC |
| Sequence-based reagent | 6xHis-FRB-f | This paper | PCR primer | atcgCGTCTCtTACGGGTCTCaA ATGggcagcagccatcatcatcatcatc acagcagcggcATCCT CTGGCATGAGATGT |
| Sequence-based reagent | EFR_icd (stop)-f | This paper | PCR primer | atcgCGTCTCtTACGGGTCT CaAGGTaagaggaaaaag aaaaacaatgcc |
| Sequence-based reagent | EFR_icd (no stop)-f | This paper | PCR primer | atcgCGTCTCtTACGGGTCTCa AGGTaagaggaaa aagaaaaacaatgcc |
| Sequence-based reagent | BAK1_icd (stop)-f | This paper | PCR primer | atcgCGTCTCtTACGGGTCTC aAGGTgcttggtggcgaagg |
| Sequence-based reagent | FLS2_icd (no stop)-f | This paper | PCR primer | atcgCGTCTCtTACGGGTCTCaAG GTACCTGTTGCAAGAAA AAAGAAAAAAAG |
| Sequence-based reagent | BRI1_icd (no stop)-f | This paper | PCR primer | atcgGGTCTCaAGGT GGTAGAGAGATGA GGAAGAGA |
| Sequence-based reagent | BRI1icd_D1009N-f | This paper | PCR primer | atcgGGTCTCaAaACATG AAATCCAGTAATGTGTTGC |
| Sequence-based reagent | EFR-r | This paper | PCR primer | cgatGAAGACttCGAAcc CATAGTATGCATGTCCG |
| Sequence-based reagent | EFR Y836F-r | This paper | PCR primer | gtgcagaAactccaaagc tgaagccacatctat |
| Sequence-based reagent | EFR SSAA-r | This paper | PCR primer | gcagcagcaaactggttt agaaaggattctcgatcg |
| Sequence-based reagent | EFR F761H-r | This paper | PCR primer | tatggcggaatgtgaaacc cacaagggtatacgacatcgt |
| Sequence-based reagent | EFR F761M-r | This paper | PCR primer | tatggcggaatgtgaaaccat gaagggtatacgacatcgta |
| Sequence-based reagent | EFR V845F-r | This paper | PCR primer | cacgttcattgtcatgaccctt tcgctcactgtgatattaagc |
| Sequence-based reagent | EFR L743F-r | This paper | PCR primer | taaactcgtcgcggttaaa gttttcaacctcctaaagc |
| Sequence-based reagent | EFR L873E-r | This paper | PCR primer | tatagagCTCctgagcca aaccaaagtcactaacatg |
| Sequence-based reagent | EFR dNLLKH-r | This paper | PCR primer | tcgtcgctcccaaaactttaa ccgcgacgagtttattct |
| Sequence-based reagent | EFR D849N-r | This paper | PCR primer | ggcttaatatTacagtgag ctacagggtcatgaca |
| Sequence-based reagent | BAK1-r | This paper | PCR primer | cgatGAAGACttAAGCc cTTATCTTGGACCCGAGG |
| Sequence-based reagent | BAK1 Y403F-r | This paper | PCR primer | catgtaaaaacgca agccctcttgcagatccca |
| Sequence-based reagent | BAK1 I338H-r | This paper | PCR primer | ccatactaTGcatctca acctctgtctggaactgc |
| Sequence-based reagent | BAK1 D416N-r | This paper | PCR primer | ctttcacatTtcgatgaata atctttgggtcgcaatg |
| Sequence-based reagent | EFRprom-r | This paper | PCR primer | atcgGgtctctCAGACGTCTC aCATTgtcgattataaaaa gataaaagaaaggtt |
| Sequence-based reagent | BAK1prom-r | This paper | PCR primer | atcgGgtctctCAGACGTCT CaCATTtttatcctcaagag attaaaaacaaac |
| Sequence-based reagent | XIIa2_Tmicd-r | This paper | PCR primer | cgatGCTCTTCtCGAAccT GAACTAGCTTCTCCTTGTG |
| Sequence-based reagent | XIIa3_Tmicd-r | This paper | PCR primer | cgatGCTCTTCtCGAAccA CGTCTGGCTGTTCTCC |
| Sequence-based reagent | XIIa4_Tmicd-r | This paper | PCR primer | cgatGCTCTTCtCGAAccAG TCTCCTCGTCTCTGAAA |
| Sequence-based reagent | XIIa5_Tmicd-r | This paper | PCR primer | cgatGCTCTTCtCGAAccACGCC AAGTCGTTCTACTGGCTT TAAAGAACCTCTCTCTGAT TGAGATCAACTCCTTG |
| Sequence-based reagent | XIIa6_Tmicd-r | This paper | PCR primer | cgatGCTCTTCtCGAAccAC GTCTAGGTGTTCTTCTG |
| Sequence-based reagent | FLS2_Tmicd-r | This paper | PCR primer | cgatGCTCTTCtCGAAccA ACTTCTCGATCCTCGTTAC |
| Sequence-based reagent | AlXIIa_D850N-r | This paper | PCR primer | atcgGCTCTTCAtACA GTGAGCTACAGGGT |
| Sequence-based reagent | BrXIIa_D846N-r | This paper | PCR primer | atcgGCTCTTCAtACAG TGAGCTATTTGGTCAT |
| Sequence-based reagent | GmXIIa_D829N-r | This paper | PCR primer | atcgGCTCTTCAtACA GTGAACTACGGCCT |
| Sequence-based reagent | PtXIIa_D848N-r | This paper | PCR primer | atcgGCTCTTCAtACa atgaatgatgggcatg |
| Sequence-based reagent | SlXIIa_D883N-r | This paper | PCR primer | atcgGCTCTTCAtACA GTGAATCATGGGTGTT |
| Sequence-based reagent | XIIa2_D803N-r | This paper | PCR primer | atcgGCTCTTCAtACAGT GAACAACTTTTACAGGTGA |
| Sequence-based reagent | XIIa3_D838N-r | This paper | PCR primer | atcgGCTCTTCATtGCAA TGAGCTATAGGCTCATGA |
| Sequence-based reagent | XIIa4_D856N-r | This paper | PCR primer | atcgGCTCTTCAtACA GTGGGCTATAGGGTTGT |
| Sequence-based reagent | XIIa5_D839N-r | This paper | PCR primer | atcgGCTCTTCAtGCAA TGAGCTATAGGTTCATGACA |
| Sequence-based reagent | XIIa6_D840N-r | This paper | PCR primer | atcgGCTCTTCAtGCAAT GAGCTATAGGCTCATGAC |
| Sequence-based reagent | FLS2icd_D997N-r | This paper | PCR primer | atcgGCTCTTCAtACAA TGAACGATGGGAAAACC |
| Sequence-based reagent | FLS2_D997N-r | This paper | PCR primer | CTTCAGATtACAATGAAC GATGGGAAAACCATATCCAGA |
| Sequence-based reagent | EFR_ecto-r | This paper | PCR primer | atcgGGTCTCacTTCT TTCTAACTGACAGAGGC |
| Sequence-based reagent | EFR_SapIdom-r | This paper | PCR primer | TGAAGCTCcTCATAACT TACCTTCTCATGGAACATCC |
| Sequence-based reagent | EFR_Esp3Idom-r | This paper | PCR primer | GAAAGAcACGGGAAGT TCTCCACTCAACATATTTGTTT |
| Sequence-based reagent | ccdB cassette-r | This paper | PCR primer | tacgGGTCTCACGAAGA AGAGCactggctgtgtataaggga |
| Sequence-based reagent | pET28_lin-r | This paper | PCR primer | ggtatatctccttct taaagttaaaca |
| Sequence-based reagent | pETGG cloning cassette-r | This paper | PCR primer | AGCAGCCGGATCTCAAAGCAGAGACCACTGGC |
| Sequence-based reagent | P641-BsaI_mut1-r | This paper | PCR primer | cagctttGgtctctcg tacggcctcctgt |
| Sequence-based reagent | P641-BsaI_mut2-r | This paper | PCR primer | agagacCactggctgtg tataagggagcctga |
| Sequence-based reagent | 6xHis-FKBP-r | This paper | PCR primer | atcgCGTCTCtCAGAGGTC TCaACCTGAGCCGCTTTCC |
| Sequence-based reagent | 6xHis-FRB-r | This paper | PCR primer | atcgCGTCTCtCAGAGGTCT CaACCTGAGCCGCTCTTT |
| Sequence-based reagent | EFR_icd (stop)-r | This paper | PCR primer | atcgCGTCTCtCAGAGGTCTC aAAGCttacatagtatgcatgtccgtatt |
| Sequence-based reagent | EFR_icd (no stop)-r | This paper | PCR primer | atcgCGTCTCtCAGAGGTCTC aAAGCttacatagtatgcatgtccgtatt |
| Sequence-based reagent | BAK1_icd (stop)-r | This paper | PCR primer | atcgCGTCTCtCAGAGGTC TCaAAGCttatcttggacccgaggg |
| Sequence-based reagent | FLS2_icd (no stop)-r | This paper | PCR primer | atcgCGTCTCtCAGAGGTCTCaC GAAccAACTTCTCGATCCTCGTTACG |
| Sequence-based reagent | BRI1_icd (no stop)-r | This paper | PCR primer | atcgGGTCTCaCGAAccTAA TTTTCCTTCAGGAACTTCTTTTATA |
| Sequence-based reagent | BRI1icd_D1009N-r | This paper | PCR primer | atcgGGTCTCaGTtTCTGT GGATGATATGCGGAC |
| Antibody | GFP-HRP; mouse monoclonal | Santa Cruz Biotechnology | Cat#: sc-9996 | Dilution: 1:1000 |
| Antibody | BAK1; rabbit polyclonal | PMID: 21693696 | Dilution: 1:10000 | |
| Antibody | BAK1 pS612; rabbit polyclonal | PMID: 30177827 | Dilution: 1:2000 | |
| Antibody | FKBP12; mouse monoclonal | Santa Cruz Biotechnology | Cat#: sc-133067 | Dilution: 1:500 |
| Antibody | p44/42; rabbit polyclonal | Cell Signaling Technology | Cat#: 9101 | Dilution: 1:1000 |
| Antibody | Anti-rabbit-HRP; goat polyclonal | Sigma-Aldrich | Cat#: A0545 | Dilution: 1:10000 |
| Antibody | Anti-mouse-HRP; goat polyclonal | Sigma-Aldrich | Cat#: A0168 | Dilution: 1:5000 |
| Recombinant DNA reagent | p641-EFRprom(BsaI) | This paper | See Materials and methods | |
| Recombinant DNA reagent | 35 S_omegaEnhancer | PMID: 21364738 | ||
| Recombinant DNA reagent | pICH41258-nMyr-FKBP | PMID: 33964457 | ||
| Recombinant DNA reagent | pICH41258-nMyr-FRB | PMID: 33964457 | ||
| Recombinant DNA reagent | pICSL01003-GFP | PMID: 21364738 | ||
| Recombinant DNA reagent | pICSL01003-mEGFP | Mark Youles, TSL | ||
| Recombinant DNA reagent | HSP18t | Mark Youles, TSL | ||
| Recombinant DNA reagent | p641-6xHis-FKBP | This paper | See Materials and methods | |
| Recombinant DNA reagent | p641-6xHis-FRB | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR Y836F | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR SSAA | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR F761H | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR F761M | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR dNLLKH | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR L743F | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR L873E | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR F761H Y836F | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR F761M Y836F | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR dNLLKH Y836F | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR L743F Y836F | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR L873E Y836F | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR D849N | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR F761H D849N | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR F761H SSAA | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL01005-EFR F761M SSAA | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICH41308-BAK1 | This paper | See Materials and MethodsSee Materials and methods | |
| Recombinant DNA reagent | pICH41308-BAK1 Y403F | This paper | See Materials and MethodsSee Materials and methods | |
| Recombinant DNA reagent | pICSL86955 | PMID: 21364738 | ||
| Recombinant DNA reagent | pICSL86955-pEFR::EFR-GFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-pEFR::EFR Y836F-GFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-pEFR::EFR SSAA-GFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-pEFR::EFR F761H-GFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-pEFR::EFR F761M-GFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-pEFR::EFR dNLLKH-GFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-pEFR::EFR L743F-GFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-pEFR::EFR L873E-GFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-pEFR::EFR F761H Y836F-GFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-pEFR::EFR F761M Y836F-GFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-pEFR::EFR dNLLKH Y836F-GFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-pEFR::EFR L743F Y836F -GFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-pEFR::EFR Y836F L873E-GFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-pEFR::EFR F761H SSAA-GFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-pEFR::EFR F761M SSAA-GFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | p641 | PMID: 31300661 | ||
| Recombinant DNA reagent | p641-EFR_icd | This paper | See Materials and methods | |
| Recombinant DNA reagent | p641-EFR F761H_icd | This paper | See Materials and methods | |
| Recombinant DNA reagent | p641-EFR D849N_icd | This paper | See Materials and methods | |
| Recombinant DNA reagent | p641-EFR F761H D849N_icd | This paper | See Materials and methods | |
| Recombinant DNA reagent | p641-BAK1_icd | This paper | See Materials and methods | |
| Recombinant DNA reagent | p641-BAK1 Y403F_icd | This paper | See Materials and methods | |
| Recombinant DNA reagent | p641-BAK1 D416N_icd | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-ccdB-GFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-XIIa2icd-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-XIIa2 D803N-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-XIIa3icd-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-XIIa3 D838N-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-XIIa4icd-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-XIIa4 D856N-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-XIIa5icd-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-XIIa5 D839N-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-XIIa6icd-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-XIIa6 D840N-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-FLS2icd-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-FLS2 D997N-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-EFRicd-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-EFR D849N-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-XA21icd-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-XA21 D803N-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-Aralyicd-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-Araly D850N-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-Brapaicd-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-Brapa D846N-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-Solycicd-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-Solyc D883N-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-Poptricd-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-Poptr D848N-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-Glymaicd-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL86955-35S::EFRecto-GlymaD829N-mEGFP::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pETGG | This paper | See Materials and methods | |
| Recombinant DNA reagent | pETGG-6xHis-FKBP-EFRicd-mEGFP | This paper | See Materials and methods | |
| Recombinant DNA reagent | pETGG-6xHis-FKBP-EFRicd D849N-mEGFP | This paper | See Materials and methods | |
| Recombinant DNA reagent | pETGG-6xHis-FKBP-BRI1-mEGFP | This paper | See Materials and methods | |
| Recombinant DNA reagent | pETGG-6xHis-FKBP-BRI1 D1009N-mEGFP | This paper | See Materials and methods | |
| Recombinant DNA reagent | pETGG-6xHis-FKBP-FLS2-mEGFP | This paper | See Materials and methods | |
| Recombinant DNA reagent | pETGG-6xHis-FKBP-FLS D997N-mEGFP | This paper | See Materials and methods | |
| Recombinant DNA reagent | pETGG-6xHis-FKBP-XIIa5-mEGFP | This paper | See Materials and methods | |
| Recombinant DNA reagent | pETGG-6xHis-FKBP-XIIa5 D839N-mEGFP | This paper | See Materials and methods | |
| Recombinant DNA reagent | pETGG-6xHis-FRB-BAK1 | This paper | See Materials and methods | |
| Recombinant DNA reagent | pET28a(+)–6xHis-BIK1 D202N | This paper | See Materials and methods | |
| Recombinant DNA reagent | pC1a1-35S::FRB-BAK1_icd::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pC1a1-35S::FRB-BAK1 Y403F_icd::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pC1a1-35S::FRB-BAK1 D416N_icd::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pC1a2-35S::FKBP-EFR_icd::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pC1a2-35S::FKBP-EFR F761H_icd::HSP18t | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL4723 | PMID: 21364738 | See Materials and methods | |
| Recombinant DNA reagent | pICSL4723-FRB-BAK1-FKBP-EFR | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL4723-FRB-BAK1 D416N-FKBP-EFR | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL4723-FRB-BAK1-FKBP-EFR F761H | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL4723-FRB-BAK1 D416N-FKBP-EFR F761H | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL4723-FRB-BAK1 Y403F-FKBP-EFR | This paper | See Materials and methods | |
| Recombinant DNA reagent | pICSL4723-FRB-BAK1 Y403F-FKBP-EFR F761H | This paper | See Materials and methods |