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| Protein | T after induction [°C] | t of expression [h] | pH of extraction buffer |
|---|---|---|---|
| For in vitro kinase assay | |||
| EFR/EFR D849N | 30 | 4 | 8.0 |
| BAK1 | 30 | 4 | 8.0 |
| BRI1/BRI1 D1009N | 18 | Over night | 8.0 |
| FLS2/FLS2 D997N | 18 | Over night | 8.0 |
| BIK1 D202N | 18 | Over night | 7.5 |
| For HDX-MS | |||
| EFR/EFR Y836F | 30 | 3 | 8.0 |
