| HDX reaction details |
20 mM HEPES, 100 mM NaCl, pD 7.4 |
20 mM HEPES, 100 mM NaCl, pD 7.4 |
| HDX time course |
0, 10, 60, 600, 3600, and 7200 s |
0, 10, 60, 600, 3600, and 7200 s |
| HDX control samples |
8 M urea-D4 for fully deuterated standard |
8 M urea-D4 for fully deuterated standard |
| Back-exchange |
46% |
49% |
| # of Peptides |
143 |
153 |
| Sequence coverage |
100% |
100% |
| Ave peptide length / Redundancy |
12.7/5.44 |
13.3/6.06 |
| Replicates |
n=3 biological repeats (3 technical/n) |
n=3 biological repeats (3 technical/n) |
| Repeatability (Ave SD) |
0.129 Da (2.28 %) |
0.111 Da (2.09 %) |
| Significant differences in HDX (98% CL) |
0.395 Da (7.18 %) |
