FIG. 10.

HIV-1 MA inhibits in vitro translation. (A) GST, wild-type GST-MA, and GST-MA dB5 (with the basic region deleted and unable to bind to EF1α) were expressed in bacteria, purified, and analyzed by Coomassie staining after SDS-PAGE, as indicated. (B) Increasing amounts of purified GST proteins (abscissa) were added to a constant amount of RRL. Luciferase-encoding mRNA was added to the reaction mixture, and the activity of the luciferase produced was used as a measure of translation efficiency (ordinate). Similar results were obtained in three separate experiments. (C) Increasing amounts of peptide encompassing the HIV-1 MA basic region or the simian virus 40 T-antigen NLS were added to a constant amount of RRL and analyzed as described above.