FIG. 1.
Expression of NSP3 in E. coli and purification of deletion mutants. (A) The cDNA encoding NSP3 from group A and group C rotavirus was cloned in pET22b+LS− and introduced in E. coli BL21(DE3). Expression of the viral proteins was induced by addition of 1 mM IPTG to the cell culture for 3 h. Whole-cell proteins were resolved by SDS-PAGE and stained with Coomassie blue. (B) Recombinant proteins were purified on an Ni2+-Sepharose column and renatured by step dialysis. Proteins were resolved by SDS-PAGE and stained with Coomassie blue. MW, molecular weight (in thousands).