FIG. 5.
Disulfide bonds in the RNA binding domain of NSP3A impair RNA binding. (A) Purified NSP3A deletions were analyzed by SDS-PAGE under reducing or nonreducing conditions and stained with Coomassie blue. (B) The NSP3A4–178 deletion mutant was UV cross-linked to the radiolabeled oligoribonucleotides indicated and analyzed by SDS-PAGE under reducing or nonreducing conditions. Autoradiograms of the gels are shown. The sizes of the molecular weight markers are indicated on the left in thousands.