FIG. 1.

Dose-dependent increase in HIV-1 LTR-driven luciferase activity. Jurkat T (A) and HeLa (B) cells were transfected with a constant amount of plasmid pwtLTR-luc2 (0.625 and 0.2 μg, respectively) and indicated amounts of plasmid pCMV-Vpr. The empty CMV expression vector was used such that each sample received an equivalent amount of the expression plasmid. A representative experiment is shown for each cell line, with fold increase in luciferase activity obtained in the presence of Vpr over the basal level of the reporter alone indicated above the histograms. Experiment was repeated three independent times with similar results. Standard errors of means are denoted by error bars.