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. Author manuscript; available in PMC: 2024 Jul 20.
Published in final edited form as: Science. 2024 Mar 21;383(6689):1312–1317. doi: 10.1126/science.adj7621

Figure 4. Domain series with additional modifying domains can be engineered into chimeric PKSs.

Figure 4

(A) Domain architecture of the chimeric PKS incorporating reducing domain series. (B) Putative structure of hydroxylated polyketide 4. (C) EICs for m/z=530.2151 of extracts of cultures of mutants expressing chimeric, reducing PKSs. The peak assigned to 4 is indicated by the shaded area. (D) Mass spectrum of the peak eluting at 12.80 minutes from culture extracts of S. plymuthica pBAD-oocQR-tar11-oocSC, showing masses and chlorination isotopes patterns corresponding to NH4+ and Na+ adducts of 4. (E) Domain architecture of the chimeric PKS incorporating dehydrating domain series. (F) Putative structure of dehydrated polyketide 5. (G) EICs for m/z=512.2046 of extracts of cultures of mutants expressing chimeric, dehydrating PKSs. (H) Mass spectra of the peaks eluting at 14.46 and 14.59 minutes from culture extracts of S. plymuthica pBAD-oocQR-lbm11DH-KR-ACP-KS-oocSC, showing masses and chlorination isotopes patterns corresponding to NH4+ and Na+ adducts of 5.