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. Author manuscript; available in PMC: 2024 Jul 20.
Published in final edited form as: Science. 2024 Mar 21;383(6689):1312–1317. doi: 10.1126/science.adj7621

Figure 5. The engineering strategy enables biosynthesis of truncated lacunalides.

Figure 5

(A) The domain architecture of the last PKSs in the lacunalide (lcn) PKS in wild type (WT) G. sunshinyii YC6258. (B) EICs for m/z values corresponding to proton adducts of compounds 6–21 for the various G. sunshinyii mutants. Each column shows the EICs obtained for the mutant mentioned above the column. The rows show the EICs obtained for m/z values indicated at the right of the row. Masses corresponding to 15 and 19 could not be observed. (C) Chemical structures of wild-type lacunalide A (6), lacunalide B (7), the NMR-confirmed truncated lacunalides 8,9, 10, 11, 12, 14, 16, 18, 20, and 21 and putative structures of truncated lacunalides 13 and 17. The shaded numbered circles indicate the moieties installed by the corresponding lcn domain series.