Fig. 5.

Nipah virus V protein prevents ISG15-induction by HIV-1. (A and B) Nipah virus V protein was expressed from a lentivector in dendritic cells (A), or macrophages (B), and cells were subsequently transduced with HIV-1-GFP for 3 d. The percentage of ISG15⁺ cells was quantified by flow cytometry and normalized to the values for cells expressing the empty vector (mean ± SEM, n = 4 donors for each). P values were determined by the two-tailed, paired t test. (C and D) Nipah virus V protein was expressed in macrophages and subsequently either infected with EMCV (C) or SeV (D). The percentage of ISG15⁺ cells was measured by flow cytometry and normalized to the values for cells expressing the empty vector (mean ± SEM, n = 3 donors for each). P values were determined by the two-tailed, paired t test.