Fig. 6.

IFIH1 mutants defective for interaction with MAVS act in trans to abrogate ISG15-induction by HIV-1 in dendritic cells. (A) Schematic of the IFIH1 domain structure and mutants generated here. (B) Dendritic cells were transduced with either the vector expressing human codon optimized WT IFIH1 or one of the three indicated IFIH1 mutants. After selection with puromycin, cells were transduced with HIV-1-GFP for 3 d. The percentage of ISG15⁺ cells was quantified by flow cytometry and normalized to the values for cells expressing WT IFIH1 (mean ± SEM, n = 3 donors for each). P values were determined by one-way ANOVA with Dunnett’s multiple comparisons test. (C) Quantification of GFP⁺ cell populations in donors used in the experiments for B, mean ± SEM, n = 3 donors.