FIG. 1.

Mapping of the optimal Mamu-A*01-restricted HIV-1 Env CTL epitope. (A) PBMC from a SHIV-HXBc2-infected rhesus monkey (L28) were cultured for 10 days with paraformaldehyde-fixed, autologous B-LCL cells infected with a vaccinia virus (vv299) expressing HIV-1 gp160. The effector cells were assayed at an E/T ratio of 10:1 with autologous B-LCL targets cultured overnight with the indicated synthetic peptides. (B) PBMC from a SHIV-HXBc2-infected rhesus monkey (L3) were cultured for 10 days with the HIV-1 gp160 20-amino-acid p41 peptide (GKAMYAPPIEGQIRCSSNIT) at a final concentration of 50 μg/ml. The effector cells were assayed at an E/T ratio of 10:1 with Mamu-A*01⁺ B-LCL targets cultured overnight with the indicated synthetic peptides. (C) Sequences of p41-derived peptides with a proline (P) at position 3.