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Mapping of the optimal Mamu-A*01-restricted SIVmac Pol CTL epitope. (A) PBMC from an SIVmac-infected rhesus monkey (GL9) were cultured for 10 days with 10 μg of the SIVmac Pol 25-amino-acid p68 peptide (WQVTWIPEWDFISTPPLVRLVFNLV) per ml. The effector cells were assayed at an E/T Ratio of 10:1 with autologous B-LCL cells cultured overnight with the indicated synthetic peptides. (B) Sequences of p68-derived peptides with a proline (P) at position 3.
