TABLE 1.

CTL specific for SIVmac Gag p11C,C-M, SIVmac Pol p68A, and HIV-1 Env p41A epitopes detectable in PBMC of SHIV- and SIVmac-infected, Mamu-A^*01⁺ rhesus monkeys after in vitro culture with peptide^a

Virus	Monkey	Gag peptide stimulated		Env peptide stimulated		Pol peptide stimulated
		% Gag tetramer-binding CD8+ T cellsb	% p11C, C-M-specific lysisc	% Env tetramer-binding CD8+ T cells	% p41A-specific lysis	% Pol tetramer-binding CD8+ T cells	% p68A-specific lysis
SHIV	L3	17.4	43.3	5.4	15.4	0.5	14.9
	L9	36.0	46.5	1.5	6.9	1.5	0.6
	206	30.6	45.0	0.7	2.3	0.0	0.0
	287	66.7	66.2	6.6	32.7	16.2	57.6
	556	25.8	48.2	1.8	14.7	1.3	5.3
SIVmac	138	8.7	61.5	NDd	ND	0.6	5.0
	357	31.2	58.7	ND	ND	1.3	26.0
	403	56.6	59.7	ND	ND	4.4	6.1
	4DK	43.1	43.1	ND	ND	9.6	37.2
	GL9	44.8	47.9	ND	ND	5.9	30.7

^aPBMC from the indicated rhesus monkeys were cultured in vitro for 10 days with 1.0 μg of the indicated optimal peptide per ml. 

^bResults are reported as percent CD3⁺ CD8αβ⁺ PBMC binding the indicated Mamu-A*01/peptide tetrameric complex. 

^cData represent percent peptide-specific lysis at an E/T ratio of 10:1. 

^dND, not done.