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. Author manuscript; available in PMC: 2024 Jul 21.
Published in final edited form as: Nat Struct Mol Biol. 2023 Dec 7;31(6):910–924. doi: 10.1038/s41594-023-01184-4

Extended Data Fig. 4. VPS35L localization and PM proteome effects.

Extended Data Fig. 4.

(A) Immunofluorescence staining for VPS35L (green channel, using HA antibody), FAM21 (red channel), and nuclei (DAPI, blue channel) in the indicated stable Huh-7 cell lines. (B) Immunofluorescence staining for VPS35L (green channel, using HA antibody), FAM21 (red channel), and nuclei (DAPI, blue channel) in the indicated HeLa knockout cell lines transfected with HA-VPS35L. (C-D) Representative gating and acquisition parameters for Villin and CD14 staining by flow cytometry.