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Target enrichment and alignment breakdown. (A) Workflow for enrichment of Illumina RNA-Seq libraries using biotinylated RNA probes designed complementary to the complete KSHV genome. (B) Enrichment protocol for full-length cDNAs for KSHV before PacBio sequencing library preparation. (C, D) Percentage of total Illumina (C) and PacBio (D) sequencing reads mapped to the KSHV reference before and after enrichment with KSHV probes.
