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. 2024 Jun 26;52(13):7720–7739. doi: 10.1093/nar/gkae540

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© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Alternate coding sequences of K8.1 and K8 proteins. (A) Alternate coding isoforms of K8.1 protein identified by TRIMD analysis. (B) Multiple sequence alignment of all K8.1 protein isoforms with each colour representing different amino acid sequences. Domains marked with same colour depict same sequence of amino acids in polypeptide chain. Numbers on the right indicate the nt. loci demarcating exon boundaries of the respective isoforms. (C) Alternate coding isoforms of K8 protein identified by TRIMD. (D) Distribution of K8.1, K8/K8.1 and RTA/K8 K8.1 encoding transcript isoforms over 19 individual TSSs with each orange bar depicting the total number of long reads (Iso-Seq read depth) founding the identification of complete transcripts originating from a TSS and the respective blue bars depicting the total numbers of complete TRIMD-identified transcripts identified from the same TSS loci. (E) Coding isoform of the LANA (ORF73) protein identified by TRIMD analysis.