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. 2024 Jun 8;52(13):7665–7686. doi: 10.1093/nar/gkae487

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© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Loss of KDM6A triggers the genomic instability in HEK293T and HeLa cells. (A) The correlation between KDM6A and DNA damage-related genes is analyzed using the dataset from GTEx database. (B) Western blot and cellular viability analysis in HEK293T and HeLa cells transfected with scramble or KDM6A shRNA plasmids. (C) Apoptosis for indicated cells with KDM6A knockdown in the presence of genotoxin CPT. (D) Cell cycle profile for indicated cells with KDM6A knockdown. (E) Abnormal chromosome in indicated cells with KDM6A knockdown. (F) Image of phospho-RPA32 (S4/S8) and γH2AX foci was captured by fluorescence microscopy. Representative images with scale bars representing 5 μm and quantifications are shown. (G) The expression of phospho-RPA32 (S4/S8 and S33) and γH2AX in HeLa cells was detected by western blot in indicated cells. The value of histogram in (B-D) and (G) represents mean data ± S.E. from three independent experiments, *P < 0.05. Significant P values are indicated and were determined using a paired Student's t test.